The United States has frozen nearly $160m (£118m) in foreign aid to Burkina Faso, the State Department announced.
After a detailed assessment of the events leading to the overthrow of President Roch Kaboré in late January, the U.S. concluded it was indeed a military coup.
The aid freeze came weeks after both the African Union and West African regional bloc ECOWAS suspended membership of Burkina Faso.
Under U.S. law, any release of funds to Burkina Faso will now be conditional on verified steps towards a return to civilian rule.
The military junta said the seizure of power from President Kaboré was over his inability to curb an Islamist insurgency that has killed thousands and forced at least 1.5 million people to flee their homes.